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產品名稱：重組大鼠遷移抑制因子蛋白
產品型號：rRtMIF
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠遷移抑制因子蛋白與其它公司提供的重組蛋白不同，rRtMIF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠遷移抑制因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠遷移抑制因子蛋白

Synonyms MIF, Glutathione-binding 13 kDa Protein, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase

Species Rat

Accession P30904

GeneID 81683

Source Escherichia coli.

Molecular Weight 重組大鼠遷移抑制因子蛋白Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.

Quantity 10μg/50μg/1000μg

AA Sequence MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Test in process.

Physical Appearance 重組大鼠遷移抑制因子蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose.

Endotoxin Less than 1 EU/μg of rRtMIF as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組大鼠遷移抑制因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠遷移抑制因子蛋白

SDS-PAGE

Reference 1. WY Weiser, PA Temple, JS Witek-Giannotti, et al. 1989. Proc Natl Acad Sci U S A, 86: 7522-6.
2. CA Kozak, MC Adamson, CE Buckler, et al. 1995. Genomics, 27: 405-11.
3. DF LarsonandK Horak. 2006. Crit Care, 10: 138.
4. HW Sun, J Bernhagen, R Bucala, et al. 1996. Proc Natl Acad Sci U S A, 93: 5191-6.
5. M Oddo, T Calandra, R Bucala, et al. 2005. Infect Immun, 73: 3783-6.
6. M Emonts, FC Sweep, N Grebenchtchikov, et al. 2007. Clin Infect Dis, 44: 1321-8.

Background Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively.

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Synonyms	MIF, Glutathione-binding 13 kDa Protein, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase
Species	Rat
Accession	P30904
GeneID	81683
Source	Escherichia coli.
Molecular Weight	重組大鼠遷移抑制因子蛋白Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.
Quantity	10μg/50μg/1000μg
AA Sequence	MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Test in process.
Physical Appearance	重組大鼠遷移抑制因子蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose.
Endotoxin	Less than 1 EU/μg of rRtMIF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組大鼠遷移抑制因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠遷移抑制因子蛋白
SDS-PAGE
Reference	1. WY Weiser, PA Temple, JS Witek-Giannotti, et al. 1989. Proc Natl Acad Sci U S A, 86: 7522-6. 2. CA Kozak, MC Adamson, CE Buckler, et al. 1995. Genomics, 27: 405-11. 3. DF LarsonandK Horak. 2006. Crit Care, 10: 138. 4. HW Sun, J Bernhagen, R Bucala, et al. 1996. Proc Natl Acad Sci U S A, 93: 5191-6. 5. M Oddo, T Calandra, R Bucala, et al. 2005. Infect Immun, 73: 3783-6. 6. M Emonts, FC Sweep, N Grebenchtchikov, et al. 2007. Clin Infect Dis, 44: 1321-8.
Background	Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively.

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產品名稱：重組大鼠遷移抑制因子蛋白