產品詳情
簡單介紹：
重組恒河猴獼猴白細胞介素3蛋白與其它公司提供的重組蛋白不同，rRhIL-3蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組恒河猴獼猴白細胞介素3蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組恒河猴獼猴白細胞介素3蛋白
| Synonyms | Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor |
| Species | Rhesus Macaque |
| Accession | P25140 |
| GeneID | 706946 |
| Source | Escherichia coli. |
| Molecular Weight | 重組恒河猴獼猴白細胞介素3蛋白Approximately 14.0 kDa, a single non-glycosylated polypeptide chain containing 124 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
重組恒河猴獼猴白細胞介素3蛋白Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 × 105 IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組恒河猴獼猴白細胞介素3蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. |
| Endotoxin | Less than 1 EU/μg of rRhIL-3 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組恒河猴獼猴白細胞介素3蛋白 | |
| Reference |
1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10. 2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95. 3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24. 4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-41. |
| Background | Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and murine IL-3 share low homology. |
