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產品名稱：重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白
產品型號：rHusTNF RII/TNFRSF1B
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白與其它公司提供的重組蛋白不同，rHusTNF RII/TNFRSF1B蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白

Species Human

Accession P20333

GeneID 7133

Source Escherichia coli.

Molecular Weight 重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2 μg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Endotoxin 重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白Less than 0.1 EU/μg of rHusTNF RII/TNFRSF1B as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白

SDS-PAGE

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Species	Human
Accession	P20333
GeneID	7133
Source	Escherichia coli.
Molecular Weight	重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2 μg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Endotoxin	重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白Less than 0.1 EU/μg of rHusTNF RII/TNFRSF1B as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白
SDS-PAGE

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產品名稱：重組人可溶性腫瘤壞死因子受體II型/ TNFRSF1B蛋白