產品詳情
簡單介紹：
重組人白介素-5蛋白與其它公司提供的重組蛋白不同，rHuIL-5蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白介素-5蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白介素-5蛋白
| Synonyms | B-cell differentiation factor I, Eosinophil differentiation factor, TRF |
| Species | Human |
| Accession | P05113 |
| GeneID | 3567 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人白介素-5蛋白Approximately 26.5 kDa, a disulfide-linked homodimeric protein containing two 116 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人白介素-5蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.5, 3 % trehalose, 0.1 % Tween-80. |
| Endotoxin | Less than 1 EU/μg of rHuIL-5 as determined by LAL method. |
| Reconstitution | 重組人白介素-5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白介素-5蛋白 | |
| SDS-PAGE |
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| Reference |
1. MV Milburn, AM Hassell, MH Lambert, et al. 1993. Nature, 363: 172-6. 2. JS Lee, HD Campbell, CA Kozak, et al. 1989. Somat Cell Mol Genet, 15: 143-52. 3. BH van Leeuwen, ME Martinson, GC Webb, et al. 1989. Blood, 73: 1142-8. 4. S Dubucquoi, P Desreumaux, A Janin, et al. 1994. J Exp Med, 179: 703-8. |
| Background | The IL-5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer. |
