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產品名稱：重組犬腫瘤壞死因子-α/ TNFSF2蛋白
產品型號：rCaTNF-α/TNFSF2
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組犬腫瘤壞死因子-α/ TNFSF2蛋白與其它公司提供的重組蛋白不同，rCaTNF-α/TNFSF2蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組犬腫瘤壞死因子-α/ TNFSF2蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組犬腫瘤壞死因子-α/ TNFSF2蛋白

Species Canine

Accession P51742

GeneID 403922

Source Escherichia coli.

Molecular Weight 重組犬腫瘤壞死因子-α/ TNFSF2蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組犬腫瘤壞死因子-α/ TNFSF2蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg in the presence of actinomycin D.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rCaTNF-α as determined by LAL method.

Reconstitution 重組犬腫瘤壞死因子-α/ TNFSF2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組犬腫瘤壞死因子-α/ TNFSF2蛋白

SDS-PAGE

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Species	Canine
Accession	P51742
GeneID	403922
Source	Escherichia coli.
Molecular Weight	重組犬腫瘤壞死因子-α/ TNFSF2蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組犬腫瘤壞死因子-α/ TNFSF2蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg in the presence of actinomycin D.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rCaTNF-α as determined by LAL method.
Reconstitution	重組犬腫瘤壞死因子-α/ TNFSF2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組犬腫瘤壞死因子-α/ TNFSF2蛋白
SDS-PAGE

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產品名稱：重組犬腫瘤壞死因子-α/ TNFSF2蛋白