產(chǎn)品詳情
簡(jiǎn)單介紹：
重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白與其它公司提供的重組蛋白不同，rRtGDNF蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白
| Synonyms | ATF |
| Species | Rat |
| Accession | Q07731 |
| GeneID | 25453 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI |
| Purity | 重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白> 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4. |
| Endotoxin | 重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白Less than 0.1 EU/μg of rRtGDNF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠膠質(zhì)細(xì)胞來(lái)源的神經(jīng)營(yíng)養(yǎng)因子蛋白 | |
| Reference |
1. Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2. 2. Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80. 3. Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7. 4. Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12. |
| Background | Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF. |
