產(chǎn)品詳情
簡單介紹：
重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白與其它公司提供的重組蛋白不同，rMuLIX/CXCL5蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白
| Synonyms | Cytokine LIX, Small-inducible Cytokine B5 |
| Species | Murine |
| Accession | P50228 |
| GeneID | 20311 |
| Source | 重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白Escherichia coli. |
| Molecular Weight | Approximately 9.8 kDa, a single, non-glycosylated polypeptide chain containing 92 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rMuLIX/CXCL5 as determined by LAL method. |
| Reconstitution | 重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組小鼠LPS誘導(dǎo)的CXC趨化因子/ CXCL5蛋白 | |
| SDS-PAGE |
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| Reference |
1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82. 2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 3. Wuyts A, Haelens A, Proost P, et al. 1996. J Immunol. 157:1736-43. 4. Wuyts A, D'Haese A, Cremers V, et al. 1999. J Immunol. 163:6155-63. |
| Background | CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) and the gene for CXCL5 is encoded by the CXCL5 gene. It May participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 (92 a.a.) contains 92 amino acids and shares 57 % amino acid sequence identity with human CXCL5. |
